The electrochemical behaviors and electrochemical kinetics of terbutaline sulfate on graphene modified glassy carbon electrode ( GR / GC) were studied and a novel electrochemical quantitative analysis for the determination of the concentration of terbutaline sulfate was establish by using electrochemical impedance spectroscopy, cyclic voltammetry, chronocoulometry, differential impulsive voltammetry and other methods. The results indicated that the electrochemical process of terbutaline sulfate on GR / GC electrode was an irreversible electrochemical oxidation, which was controlled by diffusion process. The peak current was linear to the squareroot of the scan rate in the range of 20 ~ 250 mV / s, and the process was controlled by diffusion. The method can be applied in the determination of terbutaline sulfate content in bricanyl tablets, which is simple, fast and sensitive.