Urethral catheters are indispensable medical devices for patients with urinary incontinence, urinary retention, and other urinary disorders. However, the outer surface of the catheter currently faces the problem of urinary tract infection in patients due to bacterial adherence. In addition, in clinical treatment, catheters need to be in frequent contact with body tissues either for a long or short period of time. And due to the large friction coefficient between the surface of the catheter and muscle tissues, the patient will experience varying degrees of burning pain each time when the tissues are inserted or withdrawn, which is prone to cause the corresponding mucosal damage and even tissue inflammation. Therefore, the development of catheters that are antimicrobial, inhibit bacterial aggregation and biofilm attachment, and have a lubricating effect, which is an urgent problem that needs to be solved. In order to avoid bacterial infections and friction damage complications that may occur when using urinary catheters. This paper described the development of a novel polymer brush antimicrobial coating strategy. This strategy copolymerized methacryloyloxyethyl sulfobetaine (SBMA) zwitterionic material and methacryloyloxyethyl trimethyl ammonium chloride (DMC) cationic material to form a polymer brush coating. This strategy combined the passive antimicrobial principle of resisting bacterial adhesion and the active antimicrobial principle of killing bacteria. Various PSBMA/DMC polymeric brush coatings were synthesized by a single-step polymerization process, in which the SBMA and DMC monomers were polymerized by UV-initiated grafting, and the molar ratios of SBMA and DMC in the polymerized monomer solutions were varied.