At present, as microbial corrosion has gradually become an important form of pipeline material destruction, and some traditional chemical methods to mitigate corrosion will bring various negative effects such as environmental pollution. From the angle of green environmental protection, a new green bactericidal corrosion inhibitor of our research group was selected, and the effect of the green bactericidal corrosion inhibitor on the corrosion process of carbon steel in the sulfate-reducing bacteria (SRB) and iron-oxidizing bacteria (IOB) mixed bacteria on the different surface roughness was studied to reveal the effect of the green bactericidal corrosion inhibitor on the bactericidal corrosion inhibition behavior and law of SRB+IOB biofilms on carbon steel surfaces with different roughness.