It is an advanced surface technology that can improve the biocompatibility and hemocompatibility of blood contact-like material surfaces by constructing an in situ nitric oxide (NO) catalytic release coating on the surface of 316L stainless steel (SS) without changing the physical properties of the substrate material. The biocompatibility and hemocompatibility of unmodified 316L stainless steel (SS) are too poor to cause complications such as acute thrombosis and smooth muscle cell proliferation after implantation, which can inhibit the regenerative repair of diseased tissue. Thus, in this work, a NO-catalyzed coating based on the chemical construction of catechol-(amine) surface was investigated, and a sustained and stable NO-catalyzed release from the surface of 316L SS was successfully achieved, endowing it with multiple biological functions such as anti-coagulation, selective inhibition of SMCs proliferation and promotion of EC growth.