At present, stent implantation is the main way for clinical treatment of atherosclerosis. However, after implantation, restenosis, thrombosis, inflammation and other problems may lead to treatment failure because the surface biocompatibility of implant materials does not meet the clinical requirements. Surface modification is one of the main methods to improve the biocompatibility of materials. At present, since the functional molecules modified by single function molecule can no longer meet the requirements of implant materials, the modification of multiple biomolecules on the surface of scaffolds has become a new hot spot. Therefore, the work aims to select anticoagulant molecule BVLD (Bivalirudin) and the endothelial- promoting molecule TPS (TPSLEQRTVYAK peptide) to construct BVLD/TPS modification layer on titanium surface and study the effect of TPS concentration on the biocompatibility of titanium surface, so as to obtain the optimal TPS concentration. For covalent construction of PAMAM-BVLD/TPS modification layer, titanium as a cardiovascular metal implant material, was firstly polished and then activated by NaOH to create a large number of hydroxyl reactive functional groups for electrostatic binding of positively charged PAMAM. Subsequently, according to the amino and amidation reaction of PAMAM terminal, BVLD and TPS were covalent immobilized onto surface, the PAMAM-BVLD/TPS modification layer was thus formed. Scanning electron microscopy (SEM) was used to observe the changes of the surface morphology of titanium. It was found that the surface roughness of titanium grafted with BVLD and TPS decreased. In immunofluorescence staining, the surface of the polypeptide grafted with FITC fluorescein gave off a large area of fluorescence. In FT-IR results, the successful graft of PAMAM could only be preliminarily judged. However, the successful graft of polypeptide could not be judged because the functional groups of the grafted molecules were similar. The acid orange experiment could further help to prove the successful graft of PAMAM. According to the Micro BCA experiment, the density of phenolic hydroxyl groups on the surface of the material was detected. The content of phenolic hydroxyl groups on the surface of the grafted peptide samples was significantly different from that of the contrast samples due to the unique phenolic hydroxyl group of polypeptide, proving the successful graft of the polypeptide. In the measurement of water contact Angle (WCA), the polypeptide molecule had better hydrophilicity than the other molecules, and with the increase of TPS concentration, the hydrophilicity was further improved. These experiments proved the successful construction of the BVLD/TPS modification layer. The effect of TPS concentration on the biocompatibility of the modification layer was studied in detail. In the platelet adhesion and activation experiment, the platelet activation degree on the titanium plane surface of the grafted polypeptide decreased, and the activation degree was the lowest when the TPS concentration was 3 mol/L. In the APTT experiment, the partial activation time of thrombin grafted with BVLD sample was still significantly longer than control sample, and the TPS concentration had no significant effect on the time. The APTT time of the modification layer was about 37 s, which was about 10 s longer than that of the control group. The experiment results of endothelial cell adhesion and proliferation disclosed that, compared with the control group, the number and activity of the endothelial cells adhered to the modification layer surface grafted by polypeptide increased, and the growth of the endothelial cells was excellent when the TPS concentration was 3 and 5 mol/L. Based on the above results, it was proved that the biocompatibility of modified titanium surface was optimal at the TPS concentration of 3 mol/L. Half body experiment showed that the modification layer could significantly inhibit thrombosis. Obviously, the co-immobilization of BVLD and TPS onto biomaterials surface, is expected to be used for the cardiovascular interventional materials with functionalization. BVLD/TPS modification layer is successfully constructed on titanium surface, and the optimal concentration of TPS is preliminarily explored, that is, when the concentration of TPS is 3 mol/L, its blood compatibility and cytological evaluation are good, which can be used for the development and application of biological modification layer on titanium surface.