Laser etching is an advanced surface processing technology, which can produce micro-nano surface structures with regular patterns and significantly improve the surface roughness. Compared with other laser etching, femtosecond laser etching on zirconia surface can greatly reduce thermal side effects such as thermal crack and heat-affected zone. In this paper, the zirconia surface is etched by femtosecond laser along a mesh pattern and then is modified with stearic acid. By changing the laser processing parameters, different surface micro/nano structures are obtained, and the surface morphology and wettability of the resulting zirconia samples are studied. Furthermore, the antibacterial properties of the zirconia surfaces obtained under different treatment conditions are tested and analyzed by using E.coli as test bacteria.