The work aims to synthesize the ZnTi-layered double hydroxide (LDHs) with dual functions of corrosion inhibition and antimicrobial activities. The ZnTi-LDHsintercalated with ascorbic acid (Vc) and vanillin (Van) were synthesized in one step by the reverse microemulsion method. The surface morphology, structure and composition of LDHs were characterized by scanning electron microscope (SEM), X-ray diffractometer (XRD), and Fourier transform infrared spectrometer (FTIR). The corrosion inhibition property was investigated by electrochemical impedance spectroscopy (EIS). The absorption value of bacterial suspension at 600 nm was determined by ultraviolet spectrophotometer (UV-vis, UV5200) to plot the bacteria growth curve and study the antimicrobial activities. The prepared LDHs had typical two dimensional layered structures. The XRD characteristic peaks ((003) and (006)) of LDHs after intercalated with inhibitors were obviously shifted to the high degree. The FTIR results exhibited the main characteristic peaks of Vc and Van, indicating that corrosion inhibitors were intercalated into ZnTi-LDHs successfully. The EIS results showed that the prepared LDHs exhibited excellent corrosion inhibition property for mild steel. The corrosion inhibition efficiency of ZnTi/Vc-LDHs and ZnTi/Van-LDHs reached 90.47% and 91.97% respectively after 24 hours. The bacterial growth curves showed that the prepared LDHs could inhibit the growth of staphylococcus aureus (S. aureus) and escherichia coli (E. coli) non-selectively. ZnTi/Vc-LDHs could inhibit up to 97.22% of the growth of staphylococcus aureus (S. aureus), and ZnTi/Van-LDHs could inhibit up to 98.30% of the growth of escherichia coli (E. coli) The prepared ZnTi/Vc-LDHs and ZnTi/Van-LDHs not only have good corrosion inhibition effect, but also can effectively inhibit the growth of bacteria.