The work aims to explore the influence of different ratios of B and Si elements on the amorphous formation ability of Fe-based amorphous coatings, and determine the optimum ratio of B and Si. By optimizing the ratio of B and Si elements, Fe-based amorphous coatings with uniform microstructure and excellent performance were prepared by laser cladding technology. The phase composition, microstructure, mechanical properties and tribological properties of the Fe-based amorphous coating were analyzed and tested by X-ray diffraction, scanning electron microscope, energy disperse spectroscope, Vickers indenter, nano-indentor and friction testing machine. The effects of B and Si elements on the microstructure and tribological properties of Fe-based amorphous coatings were investigated. The coating had the highest amorphous content of about 47% when the content of B and Si reached 10atm% and mainly included amorphous phase, homogeneous Fe-based solid solution and FeCrMo intermetallic compounds. The thickness of the coating was about 400 μm, and the micro-Vickers hardness was up to 1400HV0.2. Under the condition of reciprocating friction, the friction coefficient of the coating was stable at 0.45, and the wear rate was 2.28×10–6 mm3/N?m. The coating showed excellent wear resistance. The small size elements of B and Si with an atomic percentage of 10% can effectively improve the amorphous formation ability of Fe-based amorphous coating by laser cladding due to preventing the formation of FeCrMo intermetallic compounds, and further improve the hardness and tribological properties of the coating.