The work aims to study the comprehensive properties of plasma-sprayed Al2O3-3%TiO2 coating, including thermal shock resistance and load-insulation performance under external load, and explore the feasibility of using atmospheric plasma spraying to prepare insulating coatings for the magnet support structure of nuclear fusion reactor. Al2O3-3%TiO2 coating was fabricated on the grit-blasted A286 substrate by atmospheric plasma spraying and then sealing treatment was carried out to the as-sprayed coating. The microstructure and conventional propertie of the coating were studied by XRD and SEM. The low-temperature thermal shock resistance and load-insulation performance of the coating were mainly researched. The feedstock powders were melted and flattened sufficiently, and formed into typical multi-layer structure. The adhesion strength of the coating was above 30 MPa, and the porosity could be controlled below 5%. The silicone resinsealant brushed on the coating surface evenly could fill the coating pores and micro-cracks effectively, and the penetration depth of sealant was approximately 100 μm. No cracks and spallation were observed on the coating even after 10 cycles of thermal shock test from water bath at room temperature to liquid nitrogen and the thermal shock had no significant effect on the insulation performance. Under the compression load of 250 MPa, the surface resistivity of coating decreased obviously, but was still higher than 30 MΩ/sqr. Consequently, the Al2O3-3%TiO2 coating can be used as a potential material under high compression load and low-temperature environment, and atmospheric plasma spraying can become an alternative to manufacture insulating coating for the key parts in magnet support structure of nuclear fusion reactor.