Stainless steel is a widely used engineering material and improving its hydrophobic property can provide self-cleaning property and reduce corrosion. Parameters such as the size and quantity of surface morphology have an important influence on the contact angle. The work aims to construct the micro-nano structure of 3Cr13 stainless steel with hydrophobic property to analyze the relationship between the mcro-nano structure and hydrophobic property. Besides, a two-step method combining sandblasting and chemical etching was designed. The micro-nano surface of 3Cr13 stainless steel with hydrophobic property was prepared by optimizing different parameters of sand blasting and chemical etching processes. The difference between the surface microstructure of hydrophilic and hydrophobic samples was inferred from the surface morphology parameters, and the prediction was proved from the different area distribution of the microstructure. The surface of stainless steel substrate was distributed by micro-nano structures composed of micro-pores and nano-particles. Sandblasting and etching parameters affected the roughness factors on the micro-nano structure of the substrate. The size and distribution of the surface micro-pores affected the hydrophobic property. The ratio of 200~1000 μm2 pores to the total pores area on the surface of hydrophobic samples was more than 39%, while the ratio of the pores larger than 1000 μm2 to the total pores area was less than 30%. That was to say, the surface micro-structures of hydrophobic samples were mostly composed of dense small pores. By sandblasting and chemical etching, the surface of 3Cr13 stainless steel can produce micro-nano double structure with hydrophobic property, and the hydrophobic property is closely related to the area of microstructure. When the area of pore above 1000 μm2 is less than 30%, the sample is hydrophobic.