The work aims to improve the tribological properties of Fe-based amorphous coatings sprayed by detonation gun. Powder-original and powder-treated (liquid nitrogen-room temperature cycling) Fe-based amorphous coatings were deposited by detonation spraying successfully. The amorphous phases of the two coatings were measured by X-ray diffractometer (XRD). The hardness and tribological properties of the two coatings were tested by Vickers hardness tester and ball-disk friction tester, respectively. The morphology and element content in the characteristic region of the samples were characterized by scanning electron microscopy with energy dispersive spectrometer (SEM). The result shows that the powder was still in amorphous structure after cryogenic thermal cycling treatment, and no cracking and breakage occurred. Compared with the powder-original coating, the porosity of the powder-treated coating decreased from 1.0% to 0.4%. The unmelted particles decreased obviously, and the powder spreading was more sufficient. The microhardness decreased slightly from 845.4HV to 813.5HV, but the test error significantly reduced. The slope of Weibull distribution fitting line increased obviously from 7.1196 to 9.6414. The friction coefficient decreased from 0.76 to 0.73 and became more stable. The wear rate was similar, on the order of 10-6 power. Therefore, the microstructure of Fe-based powder-treated amorphous coating is more uniform and compact after cryogenic thermal cycling, the microhardness distribution is more uniform, the friction and wear properties are more stable, and the wear mechanism changes from fatigue delamination wear of the powder-original coating to dominant oxidative wear and plastic deformation.