The work aims to prepare a self-layering anticorrosive coating based on the structure and function of the lotus. Self-layering coatings were prepared by mixing polyurethane modified epoxy resin (PU/EP) and fluorosilicone modified acrylic resin (fluorosilicon modified PAA). The effects of the ratio of two resins, and the mixed solvent ratio on self-layering behavior and performance were evaluated by pencil hardness test, mechanical property test, contact angle test, aging resistance test, cross-hatch adhesion test, and EIS test, etc. The structure of the coating layer was analyzed by SEM-EDS, FTIR and other characterization techniques. When PU/EP∶fluorosilicone modified PAA was 1∶1, the contact angle reached 96.0°, the flexibility was 0.5 mm, the impact resistance was 50 cm, the adhesion level was 1, and the loss rate was reduced to 19%; When NBAC∶NBA was 4∶6, the coating film stratification was good, the contact angle reached 107.7°, water resistance had no change after immersion in water for 48 h, and the loss rate was reduced to 17%. By SEM-EDS and FTIR, the upper layer of the coating film was fluorosilicone modified PAA, the bottom layer was PU/EP, and there was a transition coating in the middle. In the transition layer, ─COOH, ─OH and epoxy groups in two reins reacted make the entire coating more stable. After immersion in 3.5% NaCl solution for 40 days, the electrolyte solution did not penetrate into the interface between the coating and the base metal by the EIS test. Therefore, the prepared rail waist bionic self-layering coating has excellent mechanical properties, adhesion, hydrophobicity, aging resistance and anti-corrosion, and the coating film structure is stable.