The work aims to prepare DLC film on the surface of 304 stainless steel and investigate the friction and wear behavior of the film in 1 mol/L NaOH, 3.5%NaCl, and 1 mol/L H2SO4 solution. DLC film was prepared by unbalanced magnetron sputtering device (UPD650). The microstructure and wear scars of DLC film were characterized by scanning electron microscopy and Raman spectroscopy. The adhesion strength, hardness and modulus of elasticity of the DLC film were tested by scratch tester and nano-indenter. The wetting angle of 1 mol/L NaOH, 3.5%NaCl, 1 mol/L H2SO4 solution, and deionized water on the surface of 304 stainless steel and DLC film were tested by contact angle meter. The friction and wear behavior of 304 stainless steel and DLC film were studied by CSM friction tester. The corrosion resistance of 304 stainless steel and DLC film were evaluated by potentiodynamic polarization. The thickness of DLC film prepared on the surface of 304 stainless steel was around 1.95 μm, the bonding force was about 37 N, and the hardness and elastic modulus were about 14.7 GPa and 191.1 GPa, respectively. Surprisingly, the coefficient of friction of the DLC film in 1 mol/L NaOH solution was as high as 0.17, while the friction coefficients in 3.5%NaCl, 1 mol/L H2SO4 solution, and deionized water were about 0.05. The wear rates of DLC film in 1 mol/L NaOH, 3.5%NaCl, and 1 mol/L H2SO4 solution were 2, 3 orders of magnitude smaller than that of 304 stainless steel. The result of polarization test showed that the order of corrosion current density was: 1 mol/L H2SO4<3.5%NaCl<1 mol/L NaOH. Therefore, the deposited DLC film processes excellent mechanical properties and corrosion resistance, which improves the wear properties of 304 stainless steel in 1 mol/L NaOH, 3.5%NaCl, and 1 mol/L H2SO4 solution.