The work aims to study the microstructure, composition and mechanical properties of NiCrWMoCuCBFe coating. The flowability and bulk density (apparent density) of the sprayed powder was characterized by Hall flowmeter. Then, NiCrWMoCuCBFe coating was prepared on 316L stainless steel substrate by High Velocity Oxygen Fuel (HVOF) spraying technique. The morphologies, microstructure and element compositions of the powder and the coating were investigated by SEM and EDS. The phases of the coating were characterized by XRD and Raman. Besides, the hardness, bonding strength and flexural strength of the coating were investigated by microhardness tester and universal material testing machine and then the fracture failure mechanism of the coating was analyzed. The NiCrWMoCuCBFe sprayed powder had great fluidity and was hardly oxidized in the process of spraying. The HVOF-sprayed NiCrWMoCuCBFe coating was very dense and exhibited typical lamellar structure. The coating was mainly composed of Ni-based solid solution and a small amount of NiO and Cr2O3 on the surface. In addition, the hardness of the coating cross section was approximately equal to that of surface which was about 600HV300g. The bonding strength between the coating and 316L stainless steel was larger than 70 MPa. When the load increased to 1800 N in the three-point bending test, the coating reached the strength limit with the strain about (4.81±0.3)%. Meanwhile, there was obvious plastic deformation. When the load remained unchanged, but the strain rose up to (11.43±0.03)%, the bonding interface between coating and 316L stainless steel substrate broke and failed. Thus, there were obvious transverse and longitudinal cracks inside the coating and the bending strength of the sample was about (1.87±0.02) GPa. HVOF-prepared NiCrWMoCuCBFe coating has excellent compactness and mechanical properties.