The work aims to study the tribological properties of PTFE film and laser-textured GCr15 bearing steel under grease lubrication conditions, and the influence of micro-texturing density and working condition on tribological behavior. Surface texture processing of lower sample using diode-pumped acousto-optic Q-switched Nd: YAG laser, and reciprocating tribological tests were carried out on a Rtec MFT-5000 multifunctional friction and wear tester. The upper sample is a cylindrical pin bonded with PTFE film, and the lower sample is a GCr15 bearing steel slider textured by laser. It is found that in grease lubrication medium, the friction coefficient for PTFE coupling with micro-textured surface is lowest；The surface friction coefficient of different micro-texture area occupancy increased slightly in the early running-in stage; In the middle stage of the experiment, the difference of surface friction coefficient between different micro-texture area occupancy gradually increased; When the area occupancy of micro-texture increases from 10% to 40%, the friction coefficient first decreases and then increases, and when the area occupancy of micro-texture is 20%, the surface friction coefficient is the smallest (only 0.032). With the increase of load from 20 N to 100 N, the friction coefficient of three samples with different micro-texture area occupancy in grease lubrication medium decreases and the trend slows down gradually. The friction coefficient is larger in the low frequency stage and smaller in the high frequency stage. It is concluded that PTFE coupling with micro-texturing is an effective anti-friction method under grease lubrication. The surface friction coefficient first decreases and then increases with the increase of texture area occupancy, the surface friction coefficient decreases rapidly with the increase of load, and the surface friction coefficient first increases and then decreases slowly with the increase of reciprocating motion frequency. When the area occupancy is 20%, better friction and lubrication properties can be obtained.