The work aims to use SLM-CoCrW as a control to evaluate the antimicrobial and biocompatibility of SLM-CoCrCu, and then provide a theoretical basis for the potential clinical application of SLM-CoCrCu. Staphylococcus aureus was selected as the research object, and the antimicrobial properties of the alloy were tested by plate method. MC3T3-E1 cells were selected as the research object. CCK-8 assay was used to evaluate the effect of alloy on cell proliferation and cytotoxicity. The adhesion and growth of cells on the alloy surface were evaluated by cytoskeleton fluorescence staining, and the cytotoxicity of the alloy was reflected by the proliferation rate. Flow cytometry was used to evaluate the effect of materials on early cell apoptosis. SLM-CoCrCu obviously inhibited the growth of staphylococcus aureus, and the antimicrobial rate of SLM-CoCrCu was 98%. There was a significant difference between SLM-CoCrCu and SLM-CoCrW (P<0.01). With the prolongation of culture time, the number of SLM-CoCrCu cells increased gradually, and promoted cell proliferation. SLM-CoCrCu and SLM-CoCrW co-cultured with cells for 1, 3 and 7 days showed significant difference in cell proliferation rate (P=0.01). After co-culture for 1 day, the cell proliferation rate of copper-free group was better than that of copper-free group. After co-culture for 3 days and 7 days, the effect of copper-containing group on cell proliferation was significantly better than that of copper-free group. There was no significant difference in cell adhesion and morphology between the two groups. There was no significant difference between SLM-CoCrCu and SLM-CoCrW on apoptosis. Compared with SLM-CoCrW, SLM-CoCrCu has better antimicrobial properties. At the same time, SLM-CoCrCu has good biocompatibility and has no significant effect on cell proliferation, adhesion and morphology.