The work aims to deposit diamond-like carbon (DLC) film on the surface of micro-arc oxidation films on titanium and explore the influence of DLC film deposition on the tribological property of micro-arc oxidation (MAO) films with different surface morphologies. Two kinds of MAO films (MAO_10 and MAO_60) with different surface roughness and micro-pore sizes were fabricated in the aluminate electrolytes with the MAO time of 10 min and 60 min, respectively. Then, a DLC film was deposited on the surface of the MAO film by magnetron sputtering to obtain MAO/DLC composite films. The surface roughness, micro-pore size, friction coefficient, wear rate and wear scar morphology of the MAO and MAO/DLC films were observed and studied by confocal microscope, CSM friction tester, Mahr surface profiler and scanning electron microscopy (SEM). The increasing MAO time increased surface roughness and average micro-pore size, but decreased micro-pore number of MAO films. After the DLC deposition, a relatively good DLC top layer was formed on the surface of MAO_10 film. However, the DLC top layer on the MAO_60 film exhibited a discontinuous distribution due to the high surface roughness and large micro-pore size of MAO_60 film. The surface roughness and average micro-pore size of MAO/DLC composite films were lower than that of MAO films. The tribological test results showed that the friction coefficient and wear rate of MAO_10/DLC composite film were significantly lower than that of MAO_10 film. The stable friction coefficient of MAO_10 film and MAO_10/DLC composite film was 0.85 and 0.24, and the wear rate was 12.76×10-6 mm3/(N?m) and 3.71×10-6 mm3/(N?m), respectively. In contrast, the stable friction coefficient and average wear rate of MAO_60/DLC composite film were near to that of MAO_60 film. The stable friction coefficient of MAO_60 film and MAO_60/DLC composite film was 0.77 and 0.67, and the wear rate was 68.02×10-6 mm3/(N?m) and 61.81×10-6 mm3/(N?m), respectively. When the DLC film is deposited on the MAO film with relatively smooth surface and small micro-pore size, the fabricated MAO/DLC composite film has good tribological property due to the formation of a continuous lubricating layer. In contrast, when the DLC film is deposited on the MAO film with rough surface and large micro-pore size, the continuous lubricating layer is difficult to form, thus leading to a relatively poor tribological property.