The work aims to construct a polypyrrole coating with the anti-bacteria adhesion performance on 304 stainless steel. Electrochemical free-template method was used to construct a polypyrrole coating with super-hydrophobic nano-cone structure on the surface of 304 stainless steel to achieve anti-bacteria adhesion. The surface morphology and chemical composition of the coating of polypyrrole were analyzed by scanning electron microscopy and Fourier transform infrared spectroscopy, respectively. Meanwhile, the elemental composition of the coating was characterized by X-ray photoelectron spectroscopy. The wettability of coating was tested by contact angle meter. The Kelvin module of the atomic force microscope was used to study the surface potential of the coating. The anti-bacterial adhesion of polypyrrole coating was studied by plate counting method, and the adhesion of bacteria on the coating surface was further observed by scanning electron microscope. Polypyrrole coating with a naphthalene sulfonic acid doped nano-cone structure was constructed on the surface of 304 by twice electrochemical polymerizations. The contact angle of the coating was 121.1°, which was highly hydrophobic. The surface potential of polypyrrole with nano-cone structure and smooth polypyrrole was (136±3) mV and (335±3) mV, respectively. E.coli adhesion experiments showed that E.coli hardly adhered to the surface of the nanoarray structure and the nanostructured polypyrrole coating had anti-bacteria adhesion properties. Nanoarray structure has stronger hydrophobicity and higher surface potential so that the repulsive force between bacteria and the surface interface is greater than the attractive force and the nano-cone structure polypyrrole has the performance of resisting bacteria adhesion.