The work aims to investigate the influence laws of initial roughness on the tribological properties of laser mi-cro-groove textured surfaces. The regularly arranged micro-grooves were fabricated on the surface of 45# steel specimens with different roughness by pulse fiber laser. The pin-to-disc reciprocating test was carried out by friction and wear tester to study the influence of initial roughness on the friction coefficient of micro-groove textured surface and the tribological behavior of laser micro-groove textured surface under different initial roughness. The friction coefficient could be significantly reduced by groove texture. The initial roughness, load and sliding velocity had significant effect on the tribological properties of groove texture. Hydrodynamic lubrication could be significantly improved by groove texture under the lower load, and boundary lubrication could be effectively improved by groove texture at higher load. There was the optimal initial roughness to minimize the friction coefficient. The optimal value of initial surface roughness could be determined according to load and sliding speed. The ideal performance of friction reduction of the textured surfaces can be obtained by optimizing the initial surface roughness according to the load and speed.