The work aims to improve the corrosion resistance and bioactivity of 316L stainless steel surface. The titanium coating was prepared on 316L by laser cladding, and then the micro-arc oxidation was carried out on the surface of the titanium layer. Therefore, porous ceramic coating containing Ca and P was prepared on the surface of 316L. The influence of the thickness of titanium layer on the surface morphology, element content and phase composition of ceramic coating was analyzed by SEM (Scanning Electron Microscope), EDS (Energy Dispersive Spectrometer) and XRD (X-Ray Diffraction). The corrosion resistance and bioactivity of the coating in 1.5× SBF solution were tested by electrochemical tests and immersion tests. Porous ceramic coating was prepared on 316L stainless steel by laser cladding and composite micro arc oxidation. The density and thickness of ceramic coating in situ formed by micro-arc oxidation increased with increase of the thickness of titanium layer. When the thickness of titanium layer reached 0.4 mm, the ceramic coating prepared by micro-arc oxidation was dense and the thickness of the dense coating reached 20 μm. The coating mainly consisted of anatase phase and rutile phase. From the polarization curve, the corrosion potential Ecorr was -0.162 V and the corrosion current density decreased to 5.11×10-7 A/cm2. After immersion in 1.5 times of SBF for 3 days, hydroxyapatite was observed on the surface of the ceramic coating. The ceramic coating prepared by laser cladding combined with micro-arc oxidation on 316L surface has excellent corrosion resistance in simulated body fluid, and can effectively improve the bioactivity of 316L stainless steel.