The work aims to discuss the effects of different substrate materials on the tribocorrosion performance of Cr/CrN alternating multilayer composite coatings in seawater, so as to provide references for the selection and application of an-ti-tribocorrosion material substrate in seawater. Cr/CrN multilayer composite coating was deposited on 316L stainless steel and TC4 titanium alloy substrate by multi-arc ion plating technique. The microstructure of coating materials was characterized by XRD and SEM and the mechanical properties, electrochemical properties and tribological properties of coating materials were analyzed by hardness test, adhesion test, electrochemical analysis, friction and wear test to compare the effects of different substrates on tribocorrosion performance of Cr/CrN multilayer coating in seawater. The hardness of the Cr/CrN multilayer coating with TC4 titanium alloy as the substrate was 1727.2HV0.3, although slightly lower than the hardness of the coating with 316L stainless steel as the substrate (2241.5HV0.3), but it was superior to the coating properties of 316L stainless steel in the membrane-base binding force, the electrochemical performance and the tribological properties of the seawater environment. The initial cracks appeared at 31 N and the extended cracks appeared at 42 N of multilayer coating with TC4 as substrate in the adhesion test, which was larger than 22 N and 35 N of 316L substrate coating. The corrosion potential of TC4 substrate coating was ?0.20 V in electrochemical test, and the corrosion potential of the 316L substrate coating was ?0.21 V. The average friction coefficient and wear rate of the TC4 substrate coating in seawater were 0.35 and 2.9950×10?5 mm3/(N?m), respectively, which were less than average friction coefficient of 0.36 and wear rate of 4.9895×10?5 mm3/(N?m) of 316L substrate coating. TC4 titanium alloy is more suitable to be used as the substrate material of Cr/CrN multilayer coating anti-tribological materials in seawater.