The work aims to study the micro-wear behavior of the NiTi alloy in Hank’s simulated body fluid. The micro-wear test was carried out on medical NiTi alloy by the TE66 micro-scale abrasion tester to calculate the wear rate and scanning electron microscopy was adopted to observe the alloy surface to analyze the wear mechanism. The effects of load, particle concentration, type and size of wear particles were considered during test. The wear rates decreased with increasing of the applied load. When the load was lower than 1.0 N, the effect of particle concentration was insignificant. When the load was higher than 1.0 N, the wear rate increased with increasing of the particle concentration. Under the same size, SiC particles had higher hardness and cutting force, so the wear rate of NiTi alloy was higher than that of Al2O3 particles. When the particles were SiC, the wear rate of NiTi alloy did not change obviously with the size of wear particle. In the experimental conditions, when the particles were SiC F1200, friction pair was ZrO2 and particle concentration was 0.05 g/cm3, the minimum wear rate of 2.3× 10?5 mm3/(N?m) was obtained. In the same concentration of wear particle, the wear rate of alloy decreases as the load increases. Under lower load, the concentration of wear particle has no significant effects on wear rate of alloy. Under higher load, the wear rate of alloy increases as the concentration of wear particle rises. The wear mechanism in this experiment is mainly the abrasive wear.