The paper aims to reduce wear of piston skirt and improve its wear resistance. The surface morphology of earthworm was taken as the bionic prototype based on the bionic non-smooth theory. A bionic through-hole structure with diameter of the hole, the distance between the holes per row and the angle of the hole along the circumference as parameters was designed in the piston skirt of LX-2V engine. Orthogonal design was applied to analyze the bionic piston with different parameters through finite element thermal structural coupling method. The maximum deformation amount of skirt, the deformation range of skirt, the stress difference between the symmetrical center line and the side point of skirt were taken as the wear resistant evaluation standards of piston skirt. Three bionic pistons and standard pistons with good wear resistance were selected through imitating method to carry outwear test of engine bench. The test results showed that the bionic through hole of reasonable structure can effectively reduce wear of the piston skirt and improve its wear resistance. Compared with the standard piston, the wear resistance of the bionic piston hole was improved by 49% when the diameter of the bionic piston hole was 5 mm, the spacing of each row hole was 6.5 mm, and the hole was 16° along the circumference angle. The maximum deformation of the skirt is too small and the deformation range of the skirt is too large. Both are not conducive to formation and maintenance of the oil film between the piston skirt and the cylinder liner, which will aggravate the wear of the skirt. Based on roughness value analysis of the piston skirt, reasonable bionic through-hole structure can optimize the surface friction of skirt. It is an important reason to further reduce the wear of piston skirt.