Nanosized TiO2 has been widely used as antibacterial materials because of its strong oxidation activity ascribed to nanometer size effects. However, TiO2 shows no antibacterial properties in dark condition, so antibacterial elements (Cu, Zn and Ag) are doped to overcome this disadvantage. TiO2 coatings mixed with Cu, Zn and Ag were produced by atmospheric plasma spraying (APS) technique. The phase composition and microstructure of the coatings were characterized by X-ray diffraction (XRD), Raman and field emission scanning electron microscopy (FE-SEM). The contact angle meter, three-position profile meter, electrochemical workstation and inductively coupled plasma atomic emission spectroscopy were adopted to analyze the hydrophilicity, surface roughness, corrosion resistance and antibacterial ion release of the coating. The antibacterial effects of coating were evaluated by the co-culture test of bacteria and coating. Doped antibacterial metal elements did not change the phase structure of the TiO2 coating and the coating was mainly composed of rutile. The coating had the surface roughness of micron order and the surface was composed of nano particles with diameter below 50 nm. The doped elements lowered the hydrophilicity of TiO2 coating. The corrosion resistance of coating mixed with Cu increased dramatically and the antibacterial metal ions could be released in various coatings and the release amount of Cu was the largest. TiO2 coating did not show antibacterial effects after being cultured with bacteria for 24 h, while the coating doped with antibacterial elements showed excellent antibacterial properties. Pre-photo irradiation by UV light could significantly improve the antibacterial function of the nanostructured TiO2 coating in the presence of H2O. The antibacterial metal elements do not change, but improves the antibacterial properties of plasma sprayed TiO2 coating significantly in dark conditions. Pre-photo irradiation by UV light can provide the non-antibacterial TiO2 coating with antibacterial properties, and can also further enhance the antibacterial properties of doped coatings.