In the long-term evolution and survival of the fittest, the biological surfaces/interfaces in nature which are covered with micro- and nanostructures oriented to the supporting layer play crucial roles in the process of spreading, climbing, locomotion, and adhesion due to the resultant frictional anisotropy. Studying the interfaces of natural orientation structure, revealing the anisotropy behavior and laws of friction, and getting inspirations to conduct construction with physical and chemical means and bionic research, are of great significance for the development of new biomimetic functional materials in many fields of modern science and technology. In this review, taking the hook-like spines of the C. Lutetiana and Galium aparine, ventral scales in the snake, and numerous keratinous setae in the gecko feet as examples, the morphology and functions of these natural biological surface/interfaces are introduced detailly, and the two mechanisms of frictional anisotropy based on the oriented structures are explained. On this foundation, the preparation methods including template and additive manufacturing and frictional performance of biomimetic surface/interface with anisotropic structures are discussed respectively. Finally, the applications of the frictional anisotropy surfaces mimicking the fast locomotion of snake and the high adhesion of gecko feet on the biomimetic driving, climbing and directional transportation are exhibited. Accordingly, the perspectives about the research emphasis and the development in the bionic frictional anisotropy field are provided.