The work aims to improve antibacterial properties of medical grade PVC surface. ZnO sol was prepared in sol-gel method, TiO2-ZnO composite suspension of different TiO2 mass fraction (20%, 40%, 60% and 80%) was prepared with P25 (commodity TiO2) suspension as doping constituent. ZnO, TiO2-ZnO and TiO2 suspensions were coated on medical-grade PVC materials uniformly in pulling method. Structure and properties of the composite film were characterized by virtue of XRD and SEM techniques. The photocatalytic antimicrobial activity and bacterial adhesion resistance of different coating materials to Escherichia coli were determined in the method of plate culture count. Photocatalytic activity of theTiO2-ZnO film doped with 20 wt% TiO2 was better than that of ZnO; photocatalytic activity of the samples doped with 40 wt%, 60 wt% and 80 wt% TiO2 was close and lower than that of the pure ZnO. ZnO as well as TiO2-ZnO composite films doped with different mass fractions of TiO2 exhibited certain bacterial adhesion resistance and antimicrobial activity. Among these samples, the sample doped with 20 wt% TiO2 exhibited best bacterial adhesion resistance. The adherent mass fraction of Escherichia coli was only 2.6%, superior to that of ZnO and TiO2 films. Under UV irradiation with wave length of 365 nm, rank of antimicrobial activity among the samples was TiO2≈20 wt% TiO2 >80 wt% TiO2>ZnO. The bacterial adhesion resistance and photocatalytic antimicrobial activity of medical-grade PVC materials can be enhanced by dip coating with ZnO sol and TiO2 suspension. Besides, TiO2-ZnO doped with 20 wt% TiO2 has the best antimicrobial activity.