The work aims to acquire an antibacterial and biological coating and improve wear and corrosion resistance of micro-arc oxidized coating on magnesium alloy. Fluorine-carrying biological coating was prepared on magnesium alloy by adding 0.4 g/L, 1.4 g/L, 2.4 g/L and 3.4 g/L NaF in plating solution by virtue of ultrasonic micro-arc oxidation technology. Effects of fluorine-carrying on surface morphology of the coatings was observed major element change of the coatings was analyzed, and coating thickness, porosity and tensile strength were determined by using scanning electron microscopy. Friction and wear test, electrochemical corrosion experiment and film-coated antibacterial experiment were performed. Binding, wear, corrosion and antibacterial properties of different fluorine-carrying biological coatings were evaluated. Holes were distributed uniformly on the surfaces of fluorine-carrying biological coating. As the NaF concentration increased, both the fluorine content and coating thickness increased, and the bonding strength of coating increased by 3.5~10.0 MPa. Fluorine could promote the formation of oxide film on coating surface and reduce the adhesive wear, hence the friction coefficient decreased by 0.17~0.35. The self-corrosion potential of fluorine-carrying coating increased by 95~170 mV while the corrosion current density decreased by about two orders of magnitude. The antibacterial rate was 61%~76%. The bonding strength, wear and corrosion resistance of fluorine-carrying biological coating are greatly improved by the addition of NaF into ultrasonic micro-arc oxidation bath, and the coating is of certain antibacterial property, which realizes the versatility of biological coating.