Objective To study the effect of the thickness and pore size of the aluminum alloy anodic oxidation film on the adhesive property. Methods Aluminum alloy anodic oxidation film was prepared. Electrolyte with 120 g/L H2SO4, 60 g/L H3PO4 and 7 g/L CH3COOH was prepared at temperature of 22 ℃. Anodized layer structures with different film thickness and different pore size were prepared by changing the anodic oxidation time and the method of anodic oxidation voltage. TS-805 adhesive coating was sprayed on anodic oxide film and then pressurized for solidification. Effects of thickness and pore size of oxide film layer on the adhesive property of anodic oxide film were evaluated by tensile shear strength test and hot and humid environment endurance test. Results Tensile shear strength increased with the increase of membrane thickness. At a certain thickness, it would not increase but decrease. When the membrane layer thickness was 9.41 μm, the tensile shear strength was up to 25.06 MPa. When the film thickness was up to a certain value, pore size influenced the tensile shear strength less. Hot and humid environment durability would be improved with the increase of oxidation time. When the oxidation time was 30 min, the hot and humid environment durability was best. It was less affected by the pore size. Conclusion Hole depth of porous layer in anodic oxidation film has an effect on bonding strength of the adhesive-bonded joint. Too thick membrane layer may cause pores on the adhesive bonding interface in the bonding process and further reduce the tensile shear strength of adhesive layer. The optimal thickness is related to the viscosity of selected adhesive layer and the pore of porous layer. Pore size has unapparent effect on the bonding shear strength. Hot and humid environment durability of the aluminum alloy is greatly related to the pore size of oxide film. For the oxide film layer structure of the same oxidation system, the larger the pore size is, the higher the hot and humid environment durability is. Oxidation voltage is the main factor to control oxide film layer structure. It can improve the hot and humid environment durability of oxide film layer by controlling the oxidation voltage.