Objective In order to match with 90Sr isotope batteries, 90Sr radioactive sources on the appointed nonmetallic material are needed. Therefore, a new preparation method of 90Sr radioactive sources was studied. Methods Dicyclohexano-18-crown-6 could not only coordinate with strontium ion but also had good resistance to radiation. The coordination technology was used to load the strontium nitrate on the crown molecule, then the product was dissolved in a functional polymer. After dropping the liquid containing strontium onto the appointed nonmetallic material, the film was dried at room temperature. The films were characterized by IR, TG, DSC, EDS and elemental analysis. Also, the binding properties between the film containing strontium and the substrate material were investigated before and after being experienced by the γ irradiation of a total dose of 116 kGy. Results The loading rate of strontium was greater than 93%. Compared with post-irradiation, the increased oscillation dropping powder rate of pre-irradiation was less than 1.3%. The results from tracing experiments showed that the relative standard deviation of the distribution of strontium in the surface of films was about 7%. Conclusion The process can obtain the strontium radioactive film on the appointed nonmetallic material, and the film is combined with substrate firmly. Strontium distribution uniformity satisfies relevant industrial standards.