Objective Polydimethylsiloxane (PDMS) was developed as commonly used polymer materials in microfluidic chip, and its own hydrophobic properties was a major obstacle to affect whole chip bonding effect. In order to effective bonding and packaging between the substrate materials, a low cost and high-efficiency PDMS material modification method was designed, which can be carried out in the general laboratory, and be successfully achieved in a short time. Methods Used ultraviolet ozone(UVO) irradiation method to study PDMS surface modification, used the orthogonal experiment method to study the UV irradiation time, distance and oxygen time on the PDMS surface modification effect, and measured the bond strength between PDMS substrate and the cover sheet (PDMS or glass) in 50 ℃ water bath at different times, and finally determined the optimal combination of the parameters. Results Compared with the traditional surface modification method of ultraviolet irradiation, for its bonding time (more than 50h) and equipment costs, this process can be successfully implemented PDMS packaging in six hours. And finally determined the P-P and P-G’s optimal parameter combinations, both average bonding strength were greater than 200KPa. Conclusion The whole process is simple, low cost, it can be used as a useful supplement to ordinary laboratories microfluidic experimental research.