Objective To improve the antimicrobial properties of near β-type biomedical titanium alloy TLM (Ti-25Nb-3Zr-2Sn-3Mo) with low modulus. Methods TLM alloy was hydrothermally treated in silver nitrate solution at 200 ℃ with AgNO3 solution for 12 h and 24 h respectively to prepare antibacterial Ag-containing TiO2 film on its surface. The microstructure and status of the film were characterized by SEM, XRD and XPS. Using the TLM alloy hydrothermally treated in deionized water as the control, the hydrophilicity of the film was compared, and the antimicrobial properties were studied using the inhibition zone method. Results SEM, XRD and XPS analyses showed that the film consisting of TiO2 nanocrystals was formed on the surface of TLM alloy after the hydrothermal treatment. X-ray photoelectron spectroscopy analysis illustrated that Ti and Nb on sample surface existed as TiO2 and Nb2 O5 respectively, and Ag was in the metallic form. Contact angle measurement indicated that the treated sample had good photoinduced hydrophilicity, and the contact angle decreased from 108° to below 10° after 30 min of UV irradiation. In the antibacterial test, obvious inhibition zones against E. coli and S. aureus were observed for the treated sample after 24 h culture, and the inhibition zone was wider against S. aureus. Conclusion Through hydrothermal treatment at 200 ℃ with 10 mmol / L of AgNO3 solution, Ag-containing TiO2 film with antimicrobial properties could be synthesized. The hydrothermal method to prepare Ag-containing TiO2 film in medical titanium alloy has the merits of easy operation, low processing temperature, evident antibacterial effect, etc.