Objective To prepare a novel green antifouling coating against marine microorganisms. Methods Acrylic resin was modified by polysiloxane (silicone), and silicone-modified acrylic resin with acrylic resin as the backbone and siloxane as the side chain was synthesized. Silica/ polydopamine/ silver (SiO2 / PD/ Ag) nanocomposites were prepared taking advantage of the adhesive and reductive properties of polydopamine. The green antifouling coating was prepared by taking the modified acrylic resin as the matrix material and mixing with silica / polydopamine / silver (SiO2 / PD / Ag) nanocomposites as bactericide. The Si—O group and contact angle of the silicone-modified acrylic resin were characterized by Fourier transform infrared spectrometer (FT-IR) and contact angle detector (CA),respectively, the preparation process of SiO2 / PD / Ag was characterized by transmission electron microscope (TEM), and the antifouling property of the coating was investigated by hydrolysis rate and bacterial adhesion. Results The introduction of Si—O—Si and Si—O—C increased the contact angle of the modified acrylic resin from 72° to 96°. SiO2 / PD / Ag is a special silver nanoparticle with " core-shell-satellite" structure, and the silver nanoparticles were evenly distributed on the surface of SiO2. The coating had a good hydrolysis performance, with a hydrolysis rate of 1. 03 μm/ d, and the bactericide was distributed evenly. Conclusion The coating had double physical antibacterial property through self-polishing and low surface energy, and the silver nanoparticles also had sterilizing property, and could therefore effectively inhibit the adhesion of marine microorganisms on the sample surface.