目的 揭示绿色杀菌缓蚀剂对不同粗糙度碳钢表面SRB+IOB生物膜的杀菌缓蚀行为与规律的影响。方法 本文采用失重实验、表面分析、电化学测试等手段，探究20#碳钢在不同粗糙度(240#、600#、1 200#)下，添加绿色杀菌缓蚀剂对混合菌的杀菌行为和对碳钢的缓蚀效果的影响，阐明D-酪氨酸分解抑制生物膜的作用机理。结果 试样表面的腐蚀产物层厚度、点蚀坑大小与深度随表面粗糙度的降低、杀菌缓蚀剂的添加，而减小。在不添加杀菌缓蚀剂情况下，碳钢随表面粗糙度从240#降到1 200#，其年腐蚀深度由0.186 mm/a降到了0.157 mm/a，下降幅度达15.6%。自腐蚀电流密度icorr由20.5 mA/cm²降到了6.93 mA/cm²，下降幅度高达66.2%。其中，在240#表面粗糙度下，碳钢年腐蚀深度由不添加杀菌缓蚀剂的0.186 mm/a降到了添加杀菌缓蚀剂的0.116 mm/a，下降幅度达37.6%。icorr由20.5 mA/cm²降到了11.8 mA/cm²，下降幅度达42.4%。结论 随表面粗糙度降低，碳钢表面趋于光滑，应力集中减小，碳钢表面很难聚集混合菌里的硫化物、矿化物，以及腐蚀性阴离子，H⁺很难与阴离子结合形成酸性环境，不易产生氧浓差电池，减缓了均匀腐蚀向局部腐蚀的转变，降低了点蚀发生概率。而D-酪氨酸的加入可将生物膜中的细菌由固着态转变为浮游态，同时破坏了细菌细胞结构、蛋白质、氧浓差环境，进而增强了杀菌缓蚀效果。

At present, as microbial corrosion has gradually become an important form of pipeline material destruction, and some traditional chemical methods to mitigate corrosion will bring various negative effects such as environmental pollution. From the angle of green environmental protection, a new green bactericidal corrosion inhibitor of our research group was selected, and the effect of the green bactericidal corrosion inhibitor on the corrosion process of carbon steel in the sulfate-reducing bacteria (SRB) and iron-oxidizing bacteria (IOB) mixed bacteria on the different surface roughness was studied to reveal the effect of the green bactericidal corrosion inhibitor on the bactericidal corrosion inhibition behavior and law of SRB+IOB biofilms on carbon steel surfaces with different roughness.