目的 在304不锈钢表面制备抗细菌粘附的聚吡咯涂层。方法 采用电化学无模板方法，在304不锈钢表面构建具有超疏水性能的纳米锥结构聚吡咯涂层，实现抗细菌粘附的功能。利用扫描电子显微镜、傅里叶红外光谱分析了聚吡咯涂层的表面形貌和化学成分，同时采用X射线光电子能谱技术表征了涂层的元素组成，采用接触角仪测试了涂层的亲疏水性，采用原子力显微镜的Kelvin探针力显微镜模块研究了涂层的表面电势，通过平板计数法研究了聚吡咯涂层的抗细菌粘附特性，并进一步通过扫描电子显微镜观察了细菌在涂层表面的粘附情况。结果 通过两次电化学聚合在304不锈钢表面构建了萘磺酸掺杂的纳米锥结构聚吡咯涂层，该涂层的接触角为121.1°，具有较强的疏水性，平滑结构聚吡咯和纳米锥结构聚吡咯的表面电势分别为(136±3) mV和(335±3) mV。大肠杆菌粘附实验结果表明，大肠杆菌几乎不粘附于纳米锥结构聚吡咯表面，纳米结构的聚吡咯涂层具有抗细菌粘附的性能。结论 纳米锥结构聚吡咯具有较强的疏水性，同时具有高的表面电势，从而使细菌与表界面的相互排斥力大于吸引力，因此纳米锥结构聚吡咯具有抗细菌粘附的性能。

The work aims to construct a polypyrrole coating with the anti-bacteria adhesion performance on 304 stainless steel. Electrochemical free-template method was used to construct a polypyrrole coating with super-hydrophobic nano-cone structure on the surface of 304 stainless steel to achieve anti-bacteria adhesion. The surface morphology and chemical composition of the coating of polypyrrole were analyzed by scanning electron microscopy and Fourier transform infrared spectroscopy, respectively. Meanwhile, the elemental composition of the coating was characterized by X-ray photoelectron spectroscopy. The wettability of coating was tested by contact angle meter. The Kelvin module of the atomic force microscope was used to study the surface potential of the coating. The anti-bacterial adhesion of polypyrrole coating was studied by plate counting method, and the adhesion of bacteria on the coating surface was further observed by scanning electron microscope. Polypyrrole coating with a naphthalene sulfonic acid doped nano-cone structure was constructed on the surface of 304 by twice electrochemical polymerizations. The contact angle of the coating was 121.1°, which was highly hydrophobic. The surface potential of polypyrrole with nano-cone structure and smooth polypyrrole was (136±3) mV and (335±3) mV, respectively. E.coli adhesion experiments showed that E.coli hardly adhered to the surface of the nanoarray structure and the nanostructured polypyrrole coating had anti-bacteria adhesion properties. Nanoarray structure has stronger hydrophobicity and higher surface potential so that the repulsive force between bacteria and the surface interface is greater than the attractive force and the nano-cone structure polypyrrole has the performance of resisting bacteria adhesion.