| XIAO Ling-yun,WU Wen-tao,ZHOU Yi-lin,YAN Zi-ni,ZHOU En-ze,SUN Ming-yue,XU Da-ke,WANG Fu-hui.Antibacterial Properties of Al0.4CoCrCuFeNi High Entropy Alloy in Living Environment[J],52(1):266-277 |
| Antibacterial Properties of Al0.4CoCrCuFeNi High Entropy Alloy in Living Environment |
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| DOI：10.16490/j.cnki.issn.1001-3660.2023.01.027 |
| KeyWord:antibacterial alloys high-entropy alloys anti-bacterial anti-fungal biofilm living-environment |
| Author | Institution |
| XIAO Ling-yun |
School of Materials Science and Engineering, Northeastern University, Shenyang , China |
| WU Wen-tao |
School of Materials Science and Engineering, Northeastern University, Shenyang , China |
| ZHOU Yi-lin |
School of Materials Science and Engineering, Northeastern University, Shenyang , China |
| YAN Zi-ni |
School of Materials Science and Engineering, Northeastern University, Shenyang , China |
| ZHOU En-ze |
School of Materials Science and Engineering, Northeastern University, Shenyang , China |
| SUN Ming-yue |
School of Materials Science and Engineering, Northeastern University, Shenyang , China |
| XU Da-ke |
School of Materials Science and Engineering, Northeastern University, Shenyang , China |
| WANG Fu-hui |
School of Materials Science and Engineering, Northeastern University, Shenyang , China |
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| Abstract: |
| By using the multi principal component design idea of high entropy alloy and adding high content of copper, the work aims to prepare the new antibacterial high-entropy alloy (AHEA, Al0.4CoCrCuFeNi) containing copper with excellent mechanical properties and to explore the antibacterial performance of it in a variety of complex living environments. The phase composition of high-entropy alloys is analyzed through XRD. Taking of the tensile test to study the mechanical properties of high entropy alloy, and collect microbial communities in various living environments. Furthermore, by plate coating, using field emission scanning electron microscope (FESEM) and confocal laser scanning microscope (CLSM) to observe the bacterial growth and film morphology, and to compare and analyze the antibacterial properties of the high entropy alloy. Through gene sequencing, OTU cluster analysis and species taxonomy analysis were carried out to statistically analyze community structure and species diversity. Through the analysis of reactive oxygen species clusters, the sterilization mechanism of high entropy alloys was explored. The gauge length of the rod tensile specimen is 33 mm and the diameter is 6 mm. A constant strain rate of the room temperature tensile test is executed 1×10‒3 s‒1. And the final yield strength result is (308±10) MPa, which is higher than that of traditional antibacterial stainless steel. In this experiment, a medical cotton swab sterilized in a sterilizer was placed in a 50 mL centrifuge tube containing 10.9 g/L PBS buffer. Three environments are randomly selected:the metal button of ATM (a), the metal door handle of laboratory (b) and the door handle of dermatology clinic (c). Then the collected strains are enriched and cultured with LB liquid medium and cultured in a shaking table at 37 ℃ for 12 hours waiting to be used. Through the calculation formula of antibacterial rate and the experimental results (R - the antibacterial rate; B - the number of viable bacteria in the control (CFU/mL); A - the number of viable bacteria in antibacterial sample (CFU/mL), it is concluded that the antibacterial rate of high entropy alloy is more than 99.99%. The results of scanning electron microscope (FESEM) analysis and live and dead staining results shows that the number of colonies on the surface of AHEA decrease significantly and there are almost no living microorganisms, cause the stained image is basically in red and it sparsely distributes. In addition, the sequencing results shows that the dominant bacteria that are easy to adhere to the surface of common metal samples are mostly harmful pathogens, including bacteria and fungi, after consulting the data, it shows that the collected dominant bacteria will cause serious diseases or have a great impact on production and life, but fortunately, AHEA inhibits them nicely. The above results indicate that AHEA can be used as a long-term broad-spectrum antibacterial material. It can prevent the reproduction and growth of bacteria and fungi on the metal surface and the formation of biofilm. The comprehensive performance is much better than that of traditional antibacterial alloys. The application prospect of copper containing high entropy alloy in daily living environment is confirmed, which provides a new idea for the further development of unique antibacterial metal materials with high antibacterial efficiency and mechanical properties. |
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