| LUO Jing,DAI Miao,LIU Xiao-ya,LI Xiao-jie.Preparation of pH-responsive Polymer Antibacterial Coating and Its Performance[J],50(10):194-204, 278 |
| Preparation of pH-responsive Polymer Antibacterial Coating and Its Performance |
| Received:January 19, 2021 Revised:April 08, 2021 |
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| DOI：10.16490/j.cnki.issn.1001-3660.2021.10.018 |
| KeyWord:antifouling coating antibacterial coating schiff base polyethylene glycol (PEG) pH response |
| Author | Institution |
| LUO Jing |
Key Laboratory of Synthetic and Biological Colloids, Ministry of Education,University, Wuxi , China;School of Chemical and Material Engineering, Jiangnan University, Wuxi , China |
| DAI Miao |
Key Laboratory of Synthetic and Biological Colloids, Ministry of Education,University, Wuxi , China;School of Chemical and Material Engineering, Jiangnan University, Wuxi , China |
| LIU Xiao-ya |
Key Laboratory of Synthetic and Biological Colloids, Ministry of Education,University, Wuxi , China;School of Chemical and Material Engineering, Jiangnan University, Wuxi , China |
| LI Xiao-jie |
Key Laboratory of Synthetic and Biological Colloids, Ministry of Education,University, Wuxi , China;School of Chemical and Material Engineering, Jiangnan University, Wuxi , China |
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| Abstract: |
| The work aims to prepare a smart polymer antibacterial coating with pH responsiveness, where the conversion of the coating function from antifouling to sterilization can be realized on demand. Firstly, methacryloxyethyl dimethyl octyl ammonium bromide (DMAEMA-8C), p-(2-methacryloxyethoxy) benzaldehyde (MAEBA) and methacrylic acid (EHA) were used as comonomer to prepare a quaternary ammonium salt polymer PMQE-CHO by free radical polymerization. And then the quaternary ammonium salt polymer coating SS-PMQE-CHO was prepared on the surface of stainless steel 316L (SS) through dip-dipping technique. Subsequently, PEG-NH2 was used to modify the surface of SS-PMQE-CHO to obtain SS-PMQE-PEG. After the basic performance of the coatings was characterized by SEM, XPS, WCA, etc, the adhesion test of E. coli and fluorescein-coupled bovine serum albumin (FITC-BSA) were used to evaluate the antifouling and antibacterial performance of the coatings. The antibacterial effect of the coatings were investigated through antibacterial experiments to verify the responsiveness in different pH conditions. And the biocompatibility of the coatings was evaluated by in vitro cytotoxicity analysis and hemolysis test. The results show that the coatings have compact and neat structure, excellent mechanical property, good biocompatibility, low hemolysis rate and effective prevention from the adhesion of BSA and E. coli. Compared with bare SS surface, the bacteria on the surface of SS-PMQE-PEG-30 and SS-PMQE-PEG-40 are respectively reduced by 94% and 96% after 10-hour incubation in the bacterial solution. At this time, PEG shows effective antifouling property. After the 24-hour culture, the pH value of bacterial solution decreases. Therefore, the functions of the coating SS-PMQE-PEG-30 and SS-PMQE- PEG-40 change from antifouling to sterilization, and the sterilization efficiency is 93% and 92%, respectively. Even after being soaked in PBS for two weeks, the coating still exhibits good functional transformation properties. Normally, the PEG in the coating SS-PMQE-PEG can effectively resist the adhesion.of protein and bacterial. When the microenvironment near the coating becomes weakly acidic, the schiff base bonds break. At this point, the bactericidal layer at the bottom of the coating is exposed to kill bacteria and suppress infection. |
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