柴应红,罗霞,林科建,张红,赵辉,蔡华伟,郝丽静.微弧氧化制备医用Mg-6Zn合金Cu2+涂层的抗肿瘤和成血管性能研究[J].表面技术,2025,54(4):201-210.
CHAI Yinghong,LUO Xia,LIN Kejian,ZHANG Hong,ZHAO Hui,CAI Huawei,HAO Lijing.Anti-tumour and Angiogenic Properties of Cu2+ Coatings on Medical Mg-6Zn Alloy Prepared by Micro-arc oxidation[J].Surface Technology,2025,54(4):201-210 |
| 微弧氧化制备医用Mg-6Zn合金Cu2+涂层的抗肿瘤和成血管性能研究 |
| Anti-tumour and Angiogenic Properties of Cu2+ Coatings on Medical Mg-6Zn Alloy Prepared by Micro-arc oxidation |
| 投稿时间：2024-06-25 修订日期：2024-11-11 |
| DOI：10.16490/j.cnki.issn.1001-3660.2025.04.016 |
| 中文关键词: 医用镁合金 微弧氧化 含Cu2+涂层 耐腐蚀性能 抗肿瘤 成血管 |
| 英文关键词:medical magnesium alloys micro-arc oxidation Cu2+-containing coatings corrosion resistance anti-tumor angiogenesis |
| 基金项目:四川省科技计划项目(2024JDHJ0003，2020);国家自然科学基金(517，52376076) |
| 作者 | 单位 |
| 柴应红 | 西南石油大学 新能源与材料学院，成都 610500 |
| 罗霞 | 西南石油大学 新能源与材料学院，成都 610500 |
| 林科建 | 成都医学院第一附属医院，成都 610599 |
| 张红 | 成都医学院第一附属医院，成都 610599 |
| 赵辉 | 西南石油大学校医院，成都 610500 |
| 蔡华伟 | 四川大学华西医院 核医学科，成都 610041 |
| 郝丽静 | 华南理工大学 国家人体组织功能重建工程技术研究中心，广州 510006 |
|
| Author | Institution |
| CHAI Yinghong | College of New Energy and Materials, Southwest Petroleum University, Chengdu 610500, China |
| LUO Xia | College of New Energy and Materials, Southwest Petroleum University, Chengdu 610500, China |
| LIN Kejian | The First Affiliated Hospital of Chengdu Medical College, Chengdu 610599, China |
| ZHANG Hong | The First Affiliated Hospital of Chengdu Medical College, Chengdu 610599, China |
| ZHAO Hui | Southwest Petroleum University Hospital, Chengdu 610500, China |
| CAI Huawei | Department of Nuclear Medicine, West China Hospital, Sichuan University, Chengdu 610041, China |
| HAO Lijing | National Engineering Research Centre for Human Tissue Function Reconstruction, South China University of Technology, Guangzhou 510006, China |
|
| 摘要点击次数: |
| 全文下载次数: |
| 中文摘要: |
| 目的 镁合金作为骨植入材料在临床上的应用受到广泛关注，然而骨植入术后的恢复问题长期以来未得到解决。方法 以Mg-6Zn合金为基体，基于简便的微弧氧化工艺，通过添加纳米铜粉，制备了含Cu2+的镁合金涂层，研究了不同纳米铜添加量下涂层的耐腐蚀、抗肿瘤和成血管性能。结果 Cu2+的加入，使涂层表面形貌得到改善，涂层更加平整致密，0.35 g/L添加量下，其拥有(385.46±38.99)HV的显微硬度、(3.90±0.28)%的孔隙率和最小(1.40±0.86) µm的孔径。在耐腐蚀性能测试中，表现出了最低的腐蚀速率(3.14´10−5 mm/a)，较不含Cu2+涂层降低了1个数量级。体外实验表明:Cu2+涂层具有良好的生物相容性，骨髓间充质干细胞的增值率大于80%，溶血率低于4%，细胞黏附效果良好。此外，Cu2+涂层还具备较好的抗肿瘤和成血管能力，培养大鼠骨肉瘤细胞时，其增值率低至(24.53±2.75)%。在合适的浓度下，Cu2+能够实现抗肿瘤和成骨的协同作用;同时，Cu2+又会促进血管内皮细胞生长因子的分泌，刺激细胞增殖分化，进而促进血管生成;而在肿瘤条件下，Cu2+又会延迟血管内皮细胞成血管。结论 Cu2+具有良好的氧化活性，会使肿瘤细胞铜过载而产生氧化应激反应，进而死亡，并且Cu2+还能上调血管内皮细胞的生长因子，促进细胞增殖进而血管化。因此，这种具备较好耐腐蚀性、抗肿瘤和成血管能力的含Cu2+医用镁合金涂层，有望在骨植入及其术后问题上得到实际应用。 |
| 英文摘要: |
| Infection and recovery problems after bone implantation have long limited the clinical application of bone implant materials such as magnesium alloys. In order to better meet the clinical needs, the work aims to prepare a Cu2+-containing magnesium alloy coating with Mg-6Zn alloy as a substrate by adding copper nanopowder based on a facile micro-arc oxidation process. The surface morphology of the coating was characterized with a ZEISS EVO MA15 scanning electron microscope and a ZEISS EVO MA15 energy spectrometer. The corrosion resistance of the sample was tested by an AUTOLAB PGSTAT302 electrochemical workstation. The value-added rate of the bone marrow mesenchymal stem cells of rats was evaluated with a Cell Counting Kit-8 kit, and the biocompatibility of the sample was assessed through observation of the adhesion morphology with a Nikon A1R laser confocal microscope. The Cell Counting Kit-8 was employed to assess the value-added rate of osteosarcoma cells in rats and observe their adhesion morphology via the Nikon A1R laser confocal microscope, thereby evaluating the anti-tumour efficacy of the sample. Calcein/PI Cell Activity A Cytotoxicity Assay Kit was employed to stain the vascular endothelial cells of rats, and a Leica inverted fluorescence microscope was used to observe their adhesion morphology, thus enabling an evaluation of the vasculogenic ability of the sample. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
渝公网安备 50010702501715号