田原,蒲亚男,孙天翔,侯苏,陈守刚.硫酸盐还原菌在顶空体积变化条件下所致镍的微生物腐蚀研究[J].表面技术,2024,53(4):68-76, 97.
TIAN Yuan,PU Yanan,SUN Tianxiang,HOU Su,CHEN Shougang.Microbiologically Influenced Corrosion of Nickel Caused by Sulfate-reducing Bacteria under Different Levels of Headspace Volume[J].Surface Technology,2024,53(4):68-76, 97 |
| 硫酸盐还原菌在顶空体积变化条件下所致镍的微生物腐蚀研究 |
| Microbiologically Influenced Corrosion of Nickel Caused by Sulfate-reducing Bacteria under Different Levels of Headspace Volume |
| 投稿时间：2022-12-22 修订日期：2023-03-30 |
| DOI：10.16490/j.cnki.issn.1001-3660.2024.04.006 |
| 中文关键词: 镍 微生物膜 硫酸盐还原菌 顶空体积 微生物腐蚀 点蚀 |
| 英文关键词:Ni biofilm sulfate-reducing bacteria headspace microbiologically influenced corrosion pitting corrosion |
| 基金项目:国家自然科学基金项目(5197229);国防科技重点实验室基金项目(JS220406) |
| 作者 | 单位 |
| 田原 | 中国海洋大学 材料科学与工程学院，山东 青岛 266100 |
| 蒲亚男 | 中国海洋大学 材料科学与工程学院，山东 青岛 266100 |
| 孙天翔 | 中国海洋大学 材料科学与工程学院，山东 青岛 266100 |
| 侯苏 | 中国海洋大学 材料科学与工程学院，山东 青岛 266100 |
| 陈守刚 | 中国海洋大学 材料科学与工程学院，山东 青岛 266100 |
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| Author | Institution |
| TIAN Yuan | School of Materials Science and Engineering, Ocean University of China, Shandong Qingdao 266100, China |
| PU Yanan | School of Materials Science and Engineering, Ocean University of China, Shandong Qingdao 266100, China |
| SUN Tianxiang | School of Materials Science and Engineering, Ocean University of China, Shandong Qingdao 266100, China |
| HOU Su | School of Materials Science and Engineering, Ocean University of China, Shandong Qingdao 266100, China |
| CHEN Shougang | School of Materials Science and Engineering, Ocean University of China, Shandong Qingdao 266100, China |
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| 中文摘要: |
| 目的 通过改变厌氧瓶内顶空体积，研究硫酸盐还原菌(SRB)对金属镍的微生物腐蚀(MIC)机理，进而为镍基合金的腐蚀防护提供依据。方法 通过生物学检测技术、表面分析技术和电化学技术，评估了金属镍的MIC行为。结果 随着顶空体积的增大，更多的H2S以气体的形式析出到顶空，液相中硫化物浓度越低，SRB浮游和固着细胞数越高，点蚀坑越深，镍的腐蚀速率越高。在200 mL的固定培养基体积下，顶空体积为90 mL和450 mL的镍试样失重分别是10 mL时的1.1倍和1.4倍，相应的点蚀坑深度分别增加了1.6倍、2.3倍。在孵育7 d后，顶空体积为450 mL时低频阻抗模值最低，同时获得最大的腐蚀电流密度，达到7.64×10–6 A.cm–2。结论 利用细胞外镍氧化释放的电子在SRB细胞质中进行硫酸盐还原在热力学上是有利的，SRB所导致的镍的腐蚀属于EET-MIC。 |
| 英文摘要: |
| Nickel (Ni), an important alloying element, has been widely added to alloys to improve their corrosion resistance, and strengthened Ni-based alloys are commonly used in industries such as oil, gas, and offshore engineering, but still face the microbiologically influenced corrosion (MIC). The volume ratio of headspace/broth can affect microbial activity, thereby affecting the metabolism of biofilm and the process of extracellular electron transfer (EET). The MIC mechanism of Ni induced by sulfate-reducing bacteria (SRB) with varied headspace volume was investigated to provide a basis for the corrosion protection of Ni-based alloys. The MIC behavior of Ni was evaluated by biological detection, surface analysis, and electrochemical analysis techniques. Different headspace volume of 10 mL, 90 mL, and 450 mL was regulated with glass bottles and glass beads of different sizes with a fixed broth volume of 200 mL. The sessile cell count of Desulfovibrio vulgaris was 107 cells/cm2 after the 3 d pre-growth. The D. vulgaris sessile cell count on Ni coupons in the anaerobic bottles with 10 mL headspace volume was 1.28×107 cell/cm2. The sessile cells count on Ni coupons in the 90 mL and 450 mL headspace volume increased by 213% and 288%, respectively compared with the 10 mL headspace. The fluorescence microscopy (FM) images also showed a relatively robust biofilm covering the Ni coupon surface. The corrosion rate of Ni coupon was analyzed by weight loss data and the maximum pitting depth distribution statistics (with a confocal laser scanning microscope, CLSM). The weight loss of Ni coupons with a headspace volume of 90 mL (1.1 mg/cm2) and 450 mL (1.4 mg/cm2) was 1.1 times and 1.4 times that of Ni coupons with the 10 mL headspace (1.0 mg/cm2), respectively. The maximum pit depth after the 7 d incubation was 2.7 µm with 10 mL headspace volume, and the corresponding pitting depth increased 1.6 times (4.2 µm) and 2.3 times (6.1 µm) for 90 mL and 450 mL headspace, respectively. With a fixed broth volume of 200 mL in all bottles, a larger headspace meant that more H2S escaped to the headspace, which reduced the toxicity of H2S in broth to D. vulgaris. The larger the headspace volume, the higher the D. vulgaris sessile cells count, the deeper the pitting pits, and the higher the corrosion rate. The lowest polarization resistance (Rp) value during the 7 d incubation period occurred in the headspace of 450 mL. Meanwhile, the lowest low-frequency resistance was obtained at a headspace volume of 450 mL. Corrosion current density (Jcorr) from potentiodynamic polarization data after the 7 d incubation with the 450 mL headspace (7.64× 10–6 A.cm–2) was more than 1.5 times and 2.1 times of that for headspace volume of 10 mL and 90 mL, respectively. The corrosion thermodynamic analysis and experimental data indicated that D. vulgaris utilized elemental Ni to replace the organic carbon source (lactate) as the electron donor. It is thermodynamically favorable to use the electrons released by extracellular Ni oxidation to reduce sulfate in D. vulgaris cytoplasm. Thus, Ni MIC by D. vulgaris belongs to EET-MIC. |
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