王梦婷,吴仲岿,代思雨.聚合物表面可控修饰构筑蛋白质检测芯片[J].表面技术,2020,49(7):9-16.
WANG Meng-ting,WU Zhong-kui,DAI Si-yu.The Protein Detection Microarray Technology Based on Controllable Modification of Polymer Surface[J].Surface Technology,2020,49(7):9-16 |
| 聚合物表面可控修饰构筑蛋白质检测芯片 |
| The Protein Detection Microarray Technology Based on Controllable Modification of Polymer Surface |
| 投稿时间：2020-06-13 修订日期：2020-07-20 |
| DOI：10.16490/j.cnki.issn.1001-3660.2020.07.002 |
| 中文关键词: 蛋白质检测芯片 探针分子 聚合物可控修饰 微图案化 蛋白质微图案 |
| 英文关键词:protein detection microarray probe molecules polymer controlled modification micropatterning protein micropattern |
| 基金项目:国家自然科学基金（50973087/E0310） |
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| 中文摘要: |
| 概述了基于聚合物表面可控修饰构筑蛋白质检测芯片的相关技术。对于探针分子筛选技术，探针分子的筛选对蛋白质芯片的灵敏度十分重要，介绍了目前常用的技术（噬菌体抗体库技术和SELEX技术）以及近年来发展的新技术（PFSC技术）的特点。聚合物表面蛋白质微图案构筑技术，可分为使用模具的模板法和基于聚合物自组装的非模板法。模板法介绍了软光刻技术和粒子束刻印技术，其特点是微图案的构筑重复精确。非模板法介绍了聚合物薄膜去湿法和呼吸图法，其特点是不依赖于刻蚀技术，表面图案形状尺寸可动态调控。对已经发表的一些该技术制备的蛋白质检测芯片及其应用方向进行了相关介绍。最后总结了该技术仍存在的不足，并针对此展望了未来研究的关注点。 |
| 英文摘要: |
| This article briefly introduces the related technologies for constructing protein microarrays based on polymer controllable modification. Probe molecular screening technology, including common technologies (such as phage antibody library technology and SELEX technology) and new technologies (PFSC technology). Construction technology of protein micropattern on polymer surface, it can be generally divided into template method using mold (such as soft lithography technology and particle beam marking technology) and non-template method based on polymer self-assembly (such as polymer film dewetting method and breath-figure method). This paper introduces some published protein detection microarrays prepared by this technology and their application directions. Finally, it summarizes the shortcomings of this technology and looks forward to the future research concerns. |
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