刘苗,谢鑫,周吴一凡,缪婉,涂秋芬,熊开琴.儿茶酚–(胺)表面化学介导的一氧化氮催化释放涂层研究[J].表面技术,2023,52(3):327-337.
LIU Miao,XIE Xin,ZHOU Wu-yi-fan,MIAO Wan,TU Qiu-fen,XIONG Kai-qin.Chemical-mediated Nitric Oxide-generating Coatings on Catechol-(Amine) Surface[J].Surface Technology,2023,52(3):327-337
儿茶酚–(胺)表面化学介导的一氧化氮催化释放涂层研究
Chemical-mediated Nitric Oxide-generating Coatings on Catechol-(Amine) Surface
  
DOI:10.16490/j.cnki.issn.1001-3660.2023.03.030
中文关键词:  表面化学  一氧化氮  抗凝血  平滑肌细胞  内皮细胞
英文关键词:surface chemistry  nitric oxide  anticoagulant  SMCs  EC
基金项目:国家自然科学基金(82072072);南方医科大学附属东莞医院高层次人才支持与发展计划(K202102);四川省国际科技创新合作项目(2021YFH0056)
作者单位
刘苗 西南交通大学 材料科学与工程学院 材料先进技术教育部重点实验室,成都 610031 
谢鑫 西南交通大学 材料科学与工程学院 材料先进技术教育部重点实验室,成都 610031 
周吴一凡 西南交通大学 材料科学与工程学院 材料先进技术教育部重点实验室,成都 610031 
缪婉 西南交通大学 材料科学与工程学院 材料先进技术教育部重点实验室,成都 610031 
涂秋芬 西南交通大学 材料科学与工程学院 材料先进技术教育部重点实验室,成都 610031 
熊开琴 西南交通大学 材料科学与工程学院 材料先进技术教育部重点实验室,成都 610031;复旦大学 聚合物分子工程国家重点实验室,上海 200438 
AuthorInstitution
LIU Miao Key Laboratory of Advanced Technology of Materials of Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China 
XIE Xin Key Laboratory of Advanced Technology of Materials of Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China 
ZHOU Wu-yi-fan Key Laboratory of Advanced Technology of Materials of Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China 
MIAO Wan Key Laboratory of Advanced Technology of Materials of Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China 
TU Qiu-fen Key Laboratory of Advanced Technology of Materials of Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China 
XIONG Kai-qin Key Laboratory of Advanced Technology of Materials of Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China;State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200438, China 
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中文摘要:
      目的 在316L不锈钢(SS)表面构建原位一氧化氮(NO)催化释放涂层,使其能特异选择性地抗凝血、抑制平滑肌细胞(SMCs)增生,从而促进内皮细胞(ECs)生长。方法 在弱碱性水溶液中,选用多巴胺(DA)和己二胺(HD)为前驱体,利用多巴胺邻二苯酚结构自聚合沉膜的能力、多巴胺与己二胺的酚–(胺)表面化学,通过简单的一锅法在316L SS表面构建富氨基粘附涂层DA/HD。再通过碳二亚胺化学交联反应,共价接枝螯合Cu2+的1, 4, 7, 10–四氮杂环十二烷–1, 4, 7, 10–四羧酸(DOTA),最后获得均匀且稳定的NO催化释放涂层(命名为Cu-DOTA@DA/HD)。结果 DA/HD涂层表面的氨基密度高达22 nmol/cm2,实现了Cu-DOTA的有效固定,其NO催化释放速率可达5.2×10–10 mol/(cm2.min)。Cu-DOTA@DA/HD涂层显著地抑制了血小板的粘附和激活,也能有效抑制血栓的形成,其表面血栓总质量由316L SS的(40.3±10.3) mg降低至(3.0±0.4) mg。Cu-DOTA@DA/HD涂层催化释放的NO赋予了316L SS选择性地抑制SMCs粘附和增殖,从而促进了ECs生长的功能。结论 基于儿茶酚–(胺)表面化学构建的NO催化涂层,成功地实现了316L SS表面持续、稳定的NO催化释放,赋予了其抗凝血、选择性抑制SMCs增殖和促进EC生长等多重生物学功能,在血液接触类器械(如体外循环导管和血管支架表面)应用方面具有潜在价值。
英文摘要:
      It is an advanced surface technology that can improve the biocompatibility and hemocompatibility of blood contact-like material surfaces by constructing an in situ nitric oxide (NO) catalytic release coating on the surface of 316L stainless steel (SS) without changing the physical properties of the substrate material. The biocompatibility and hemocompatibility of unmodified 316L stainless steel (SS) are too poor to cause complications such as acute thrombosis and smooth muscle cell proliferation after implantation, which can inhibit the regenerative repair of diseased tissue. Thus, in this work, a NO-catalyzed coating based on the chemical construction of catechol-(amine) surface was investigated, and a sustained and stable NO-catalyzed release from the surface of 316L SS was successfully achieved, endowing it with multiple biological functions such as anti-coagulation, selective inhibition of SMCs proliferation and promotion of EC growth.
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