肖凌云,武文韬,周沂霖,闫姿霓,周恩泽,孙明月,徐大可,王福会.Al0.4CoCrCuFeNi高熵合金在生活环境中的抗菌性能[J].表面技术,2023,52(1):266-277.
XIAO Ling-yun,WU Wen-tao,ZHOU Yi-lin,YAN Zi-ni,ZHOU En-ze,SUN Ming-yue,XU Da-ke,WANG Fu-hui.Antibacterial Properties of Al0.4CoCrCuFeNi High Entropy Alloy in Living Environment[J].Surface Technology,2023,52(1):266-277
Al0.4CoCrCuFeNi高熵合金在生活环境中的抗菌性能
Antibacterial Properties of Al0.4CoCrCuFeNi High Entropy Alloy in Living Environment
  
DOI:10.16490/j.cnki.issn.1001-3660.2023.01.027
中文关键词:  抗菌材料  高熵合金  抗细菌  抗真菌  生物被膜  生活环境
英文关键词:antibacterial alloys  high-entropy alloys  anti-bacterial  anti-fungal  biofilm  living-environment
基金项目:辽宁省兴辽英才计划项目(XLYC1907158)
作者单位
肖凌云 东北大学 材料科学与工程学院,沈阳 110819 
武文韬 东北大学 材料科学与工程学院,沈阳 110819 
周沂霖 东北大学 材料科学与工程学院,沈阳 110819 
闫姿霓 东北大学 材料科学与工程学院,沈阳 110819 
周恩泽 东北大学 材料科学与工程学院,沈阳 110819 
孙明月 东北大学 材料科学与工程学院,沈阳 110819 
徐大可 东北大学 材料科学与工程学院,沈阳 110819 
王福会 东北大学 材料科学与工程学院,沈阳 110819 
AuthorInstitution
XIAO Ling-yun School of Materials Science and Engineering, Northeastern University, Shenyang 110819, China 
WU Wen-tao School of Materials Science and Engineering, Northeastern University, Shenyang 110819, China 
ZHOU Yi-lin School of Materials Science and Engineering, Northeastern University, Shenyang 110819, China 
YAN Zi-ni School of Materials Science and Engineering, Northeastern University, Shenyang 110819, China 
ZHOU En-ze School of Materials Science and Engineering, Northeastern University, Shenyang 110819, China 
SUN Ming-yue School of Materials Science and Engineering, Northeastern University, Shenyang 110819, China 
XU Da-ke School of Materials Science and Engineering, Northeastern University, Shenyang 110819, China 
WANG Fu-hui School of Materials Science and Engineering, Northeastern University, Shenyang 110819, China 
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中文摘要:
      目的 利用高熵合金多主元的设计思想,添加高含量的铜制备出具有优良机械性能的新型含铜抗菌高熵合金(Antibacterial High-entropy Alloy),探究其在多种复杂生活环境中服役时的抗菌性能。方法 通过XRD分析高熵合金的物相组成。通过拉伸试验研究该高熵合金的机械性能。采集多种生活环境下的微生物群落,通过平板涂布、场发射扫描电子显微镜和激光共聚焦显微镜观察细菌生长与成膜形貌,对比分析高熵合金的抗菌性能。通过基因测序,经由OTU聚类分析与物种分类学分析等研究手段,统计和分析菌落结构与物种多样性。通过活性氧簇分析,探究高熵合金的杀菌机理。结果 Al0.4CoCrCuFeNi高熵合金的屈服强度为(308±10) MPa,高于传统抗菌不锈钢材料。通过抗菌试验结果得出,高熵合金的抗菌率达到99.99%以上。通过扫描电镜分析和活死染色结果,发现AHEA表面菌落数量显著减少且几乎没有任何活的微生物。此外,通过测序结果可以看出,易黏附于普通金属样品表面的优势菌种多为有害致病菌,包含细菌和真菌,而AHEA对它们有着良好的抑制作用。结论 AHEA可以作为一种高效的抗菌材料,不仅能防止细菌在金属材料表面繁殖生长,还能有效抑制真菌生长和生物被膜的形成,综合性能远优于传统抗菌合金。
英文摘要:
      By using the multi principal component design idea of high entropy alloy and adding high content of copper, the work aims to prepare the new antibacterial high-entropy alloy (AHEA, Al0.4CoCrCuFeNi) containing copper with excellent mechanical properties and to explore the antibacterial performance of it in a variety of complex living environments. The phase composition of high-entropy alloys is analyzed through XRD. Taking of the tensile test to study the mechanical properties of high entropy alloy, and collect microbial communities in various living environments. Furthermore, by plate coating, using field emission scanning electron microscope (FESEM) and confocal laser scanning microscope (CLSM) to observe the bacterial growth and film morphology, and to compare and analyze the antibacterial properties of the high entropy alloy. Through gene sequencing, OTU cluster analysis and species taxonomy analysis were carried out to statistically analyze community structure and species diversity. Through the analysis of reactive oxygen species clusters, the sterilization mechanism of high entropy alloys was explored. The gauge length of the rod tensile specimen is 33 mm and the diameter is 6 mm. A constant strain rate of the room temperature tensile test is executed 1×10‒3 s‒1. And the final yield strength result is (308±10) MPa, which is higher than that of traditional antibacterial stainless steel. In this experiment, a medical cotton swab sterilized in a sterilizer was placed in a 50 mL centrifuge tube containing 10.9 g/L PBS buffer. Three environments are randomly selected:the metal button of ATM (a), the metal door handle of laboratory (b) and the door handle of dermatology clinic (c). Then the collected strains are enriched and cultured with LB liquid medium and cultured in a shaking table at 37 ℃ for 12 hours waiting to be used. Through the calculation formula of antibacterial rate and the experimental results (R - the antibacterial rate; B - the number of viable bacteria in the control (CFU/mL); A - the number of viable bacteria in antibacterial sample (CFU/mL), it is concluded that the antibacterial rate of high entropy alloy is more than 99.99%. The results of scanning electron microscope (FESEM) analysis and live and dead staining results shows that the number of colonies on the surface of AHEA decrease significantly and there are almost no living microorganisms, cause the stained image is basically in red and it sparsely distributes. In addition, the sequencing results shows that the dominant bacteria that are easy to adhere to the surface of common metal samples are mostly harmful pathogens, including bacteria and fungi, after consulting the data, it shows that the collected dominant bacteria will cause serious diseases or have a great impact on production and life, but fortunately, AHEA inhibits them nicely. The above results indicate that AHEA can be used as a long-term broad-spectrum antibacterial material. It can prevent the reproduction and growth of bacteria and fungi on the metal surface and the formation of biofilm. The comprehensive performance is much better than that of traditional antibacterial alloys. The application prospect of copper containing high entropy alloy in daily living environment is confirmed, which provides a new idea for the further development of unique antibacterial metal materials with high antibacterial efficiency and mechanical properties.
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