彭兰,张国芬,罗迪迪,王琴,甘英睿,朱玉发,刘其斌.不同wCa/wP对宽带激光熔覆生物陶瓷涂层性能的影响[J].表面技术,2022,51(7):363-369.
PENG Lan,ZHANG Guo-fen,LUO Di-di,WANG Qin,GAN Ying-rui,ZHU Yu-fa,LIU Qi-bin.Effects of Different Ca/P on Properties of Bioceramic Coating Prepared by Broadband Laser Cladding[J].Surface Technology,2022,51(7):363-369 |
| 不同wCa/wP对宽带激光熔覆生物陶瓷涂层性能的影响 |
| Effects of Different Ca/P on Properties of Bioceramic Coating Prepared by Broadband Laser Cladding |
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| DOI：10.16490/j.cnki.issn.1001-3660.2022.07.036 |
| 中文关键词: 激光熔覆 Ca/P涂层 微观组织 生物相容性 生物活性 |
| 英文关键词:laser cladding Ca-P coating microstructure biocompatibility bioactivity |
| 基金项目: |
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| Author | Institution |
| PENG Lan | College of materials and metallurgy, Guizhou University, Guizhou Guiyang 550025, China |
| ZHANG Guo-fen | College of materials and metallurgy, Guizhou University, Guizhou Guiyang 550025, China |
| LUO Di-di | College of materials and metallurgy, Guizhou University, Guizhou Guiyang 550025, China |
| WANG Qin | College of materials and metallurgy, Guizhou University, Guizhou Guiyang 550025, China |
| GAN Ying-rui | College of materials and metallurgy, Guizhou University, Guizhou Guiyang 550025, China |
| ZHU Yu-fa | College of materials and metallurgy, Guizhou University, Guizhou Guiyang 550025, China |
| LIU Qi-bin | College of materials and metallurgy, Guizhou University, Guizhou Guiyang 550025, China |
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| 中文摘要: |
| 目的 对不同wCa/wP的宽带激光熔覆生物陶瓷涂层性能进行研究，探究wCa/wP对涂层硬度、生物相容性及生物活性的影响规律，寻求最优试验参数，为以后制备性能优良的涂层提供参考。方法 运用梯度设计思想，采用宽带激光熔覆技术，在钛合金表面分别制备wCa/wP为1.35、1.40、1.45的梯度生物陶瓷涂层。利用X射线衍射仪(XRD)、扫描电镜(SEM)、金相显微镜、显微硬度仪、模拟体液浸泡试验、体外细胞试验，研究不同wCa/wP对生物陶瓷涂层组织结构、生物相容性以及生物活性的影响。结果 XRD结果表明，涂层主要由CaTiO3、HA、Ca2SiO4、CaO、TCP等相组成。硬度测试表明，wCa/wP=1.45的涂层平均显微硬度最高，而wCa/wP=1.35的涂层平均硬度最低。体外细胞试验表明，所有涂层上的MG63分布均匀，细胞形态良好，wCa/wP=1.40的涂层上的OD值增长较快，共同培养至第5天时，测得的OD值远高于其他涂层，相较于第1天的OD值增加最多。SBF浸泡试验表明，3种wCa/wP的陶瓷涂层都在表面覆盖了类骨磷灰石，wCa/wP=1.40的涂层，经浸泡后表面覆盖了最多的类骨磷灰石。观察细胞生长形态，细胞的许多丝状伪足紧贴在涂层上，使得细胞可以粘附在涂层上并生长，呈现出正常的梭形形态，具备较完整的细胞膜，wCa/wP=1.40的涂层上粘附的细胞数量更多、分布更为均匀。结论 当wCa/wP=1.40时，Ca-P生物陶瓷涂层不仅拥有良好的生物矿化能力，能够形成更多类骨磷灰石，而且涂层上增殖吸附的细胞数量最多，涂层表现出良好的生物相容性和生物活性。 |
| 英文摘要: |
| This paper aims to study the performance of broadband laser cladding bioceramic coatings with different wCa/wP, explore the influence of wCa/wP on coating hardness, biocompatibility and biological activity and seek the optimal experimental parameters to provide a reference for the future preparation of coatings with excellent performance. In biomedical implant materials, bioceramic coatings prepared by laser cladding technology on titanium alloy surface have attracted wide attention due to their excellent mechanical properties and osteogenic properties. At present, relevant studies only focus on the influence of La2O3 and SiO2 doping amount on the formation of HA+TCP and coating biological properties, and do not involve the influence of different wCa/wP on coating properties. Therefore, this paper adopts the broadband laser cladding technology and the composition gradient design idea. The titanium alloy Ti-6Al-4V is used as the matrix material, the coating material is CaHPO4.2H2O, CaCO3 and Ti powder, and the doping materials are selected as 0.6wt.% La2O3 and 10wt.% SiO2. This paper adopted to gradient design idea, using broadband laser cladding technology. Gradient bioceramic coatings with wCa/wP of 1.35, 1.40 and 1.45 were prepared. And by using X-ray diffractometer (XRD), scanning electron microscope (SEM), metallographic microscope, microhardness tester, simulated body fluid immersion test, in vitro cell test, system research different wCa/wP of biological ceramic coating structure, the effects of different wCa/wP on the coating structure, biocompatibility and bioactivity of bioceramic coatings were studied. the influence of the biocompatibility and bio activity, to seek the optimal experimental parameters, provide a reference for future preparation of high performance coating. XRD shows that the coating is mainly composed of CaTiO3, HA, Ca2SiO4, CaO and TCP phases. When wCa/wP=1.40, the number of HA+TCP is the largest. Hardness test shows that the higher the wCa/wP is, the higher the average hardness of the coating is. The coating with wCa/wP=1.45 has fewer cracks and the highest average microhardness, while the coating with wCa/wP=1.35 has the lowest average hardness. In vitro cell experiments showed that MG63 cells on all the coatings were evenly distributed and had good growth morphology, indicating that all the coatings had no toxicity to cells. Among them, The OD value of the coating with wCa/wP=1.40 increased rapidly, and the OD value measured on the fifth day of co-culture was much higher than that of other coatings, and the OD value increased the most compared with that on the first day. SBF solution immersion experiment showed that the three wCa/wP ceramic coatings were covered with osteoid apatite on the surface. The coating with wCa/wP=1.40 was covered with the most osteoid apatite after soaking. It was found that many filamentous pseudopods of the cells were closely attached to the coating, so that the cells could adhere to the coating and grow, and showed a normal spindle shape with relatively complete cell membrane. The coating with wCa/wP=1.40 adhered to more cells and distributed more evenly. In conclusion, when wCa/wP=1.40, wCa/wP bioceramic coating not only has good biomineralization ability and can form more bone-like apatite, but also has the largest number of cells proliferated and adsorbed on the coating, showing good bioactivity and biocompatibility. |
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