刘倩倩,卢琳,肖葵.热带雨林环境中涂镀层表面的真菌群落研究[J].表面技术,2021,50(7):295-309.
LIU Qian-qian,LU Lin,XIAO Kui.Study on Fungi Community of Coating Surface in Tropical Rainforest[J].Surface Technology,2021,50(7):295-309
热带雨林环境中涂镀层表面的真菌群落研究
Study on Fungi Community of Coating Surface in Tropical Rainforest
投稿时间:2020-09-25  修订日期:2021-02-09
DOI:10.16490/j.cnki.issn.1001-3660.2021.07.031
中文关键词:  热带雨林  真菌多样性  鉴定  涂层老化  微生物腐蚀
英文关键词:tropical rainforest  microbial diversity  identification  coating aging  microbiologically influenced corrosion
基金项目:国家自然科学基金(52071019,51971032);国家重点研发计划(2018YFB0605502);自然科学基金钢铁联合基金(U1560104)
作者单位
刘倩倩 北京科技大学 新材料技术研究院,北京 100083 
卢琳 北京科技大学 新材料技术研究院,北京 100083;海洋装备用金属材料及其应用国家重点实验室,辽宁 鞍山 114000 
肖葵 北京科技大学 新材料技术研究院,北京 100083 
AuthorInstitution
LIU Qian-qian Institute of Advanced Materials and Technology, University of Science and Technology Beijing, Beijing 100083, China 
LU Lin Institute of Advanced Materials and Technology, University of Science and Technology Beijing, Beijing 100083, China;State Key Laboratory of Metal Material for Marine Equipment and Application, Anshan 114000, China 
XIAO Kui Institute of Advanced Materials and Technology, University of Science and Technology Beijing, Beijing 100083, China 
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中文摘要:
      目的 研究热带雨林大气环境中5种常用涂(镀)层钢板表面的菌群分布及其变化规律,并深入讨论真菌对涂镀层材料的破坏作用。方法 对聚酯涂层、氟碳涂层、含铬耐指纹涂层、无铬耐指纹涂层和热镀锌板在内的5种材料,在西双版纳大气试验站进行2年的大气暴晒实验,干季和雨季共进行4次菌种采集。结合传统的培养法和现代分子生物学的方法,鉴定涂镀层材料表面生长的真菌,并对优势菌进行统计学分析。通过扫描电子显微镜(SEM)和能谱(EDS)研究霉菌对涂镀层材料的腐蚀行为。结果 从投试材料表面共分离纯化了真菌154株。根据ITS rDNA系统发育分析,鉴定为子囊菌门(Ascomycota)的3纲10目37属和担子菌门(Basidiomycota)的3纲6目12属。用统计学法得出,轮层炭菌属(Daldinia)、炭角菌属(Xylaria)、曲霉属(Aspergillus)、篮状菌属(Talaromyces)、弯孢属(Curvularia)、鬼伞属(Coprinellus)、链格孢属(Alternaria)、黑团孢属(Periconia)、拟鬼伞属(Coprinopsis)和皮司霉属(Pithomyces)等在数量、分布和季节上均为优势菌属。结论 以炭角菌属、弯孢属和曲霉属等为代表的真菌生命活动可能与涂层的劣化过程具有较强的相关性,可考虑作为相关材料霉菌实验的添加菌种。
英文摘要:
      In this study, our object is to investigate the fungal community structure and diversity on different coating materials in the tropical rainforest, and to discuss the corrosion or biodegradation effect of fungi.Five materials including polyester coating, fluorocarbon coating, Cr-containing anti-fingerprint coating, Cr-free anti-fingerprint coating and hot-dip galvanized sheet were exposed 2 years at Xishuangbanna atmosphere test station. We collected the fungi from the materials in dry and rainy seasons for 4 times, which were cultured by traditional culture methods and identified by molecular biology methods. The dominant genera were statistically analyzed. Scanning electron microscope (SEM) and energy dispersive spectroscopy (EDS) were used to study the corrosion behavior of molds on the coatings. Finally, 154 strains of fungi were isolated and purified. Of them, 37 genera in 10 orders of 3 classes of Ascomycota and 12 genera in 6 orders of 3 classes of Basidiomycota were identified by ITS rDNA sequence analysis. The dominant genera based on statistics were Daldinia, Xylaria, Aspergillus, Talaromyces, Curvularia, Coprinellus, Alternaria, Periconia, Coprinopsis, and Pithomyces. The life activities of the dominant genera, especially Xylaria, Curvularia, and Aspergillus may have a strong correlation with the coatings deterioration process, which can be used in the indoor fungus test.
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