吴倩,许升,刘雅媛,李小杰,刘晓亚.光固化复合电泳涂层材料的制备及其传感性能[J].表面技术,2019,48(6):180-188.
WU Qian,XU Sheng,LIU Ya-yuan,LI Xiao-jie,LIU Xiao-ya.Preparation and Sensing Properties of Photocurable Composite Electrophoretic Coating[J].Surface Technology,2019,48(6):180-188 |
| 光固化复合电泳涂层材料的制备及其传感性能 |
| Preparation and Sensing Properties of Photocurable Composite Electrophoretic Coating |
| 投稿时间：2018-11-14 修订日期：2019-06-20 |
| DOI：10.16490/j.cnki.issn.1001-3660.2019.06.021 |
| 中文关键词: 静电自组装 光交联 电泳沉积 涂层 生物传感器 氧化石墨烯 |
| 英文关键词:electrostatic self-assembly photo-cross linking electrophoretic deposition coating, biological sensor GO |
| 基金项目:国家自然科学基金项目（51573073） |
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| Author | Institution |
| WU Qian | School of Material Science and Engineering, Jiangnan University, Wuxi 214122, China |
| XU Sheng | School of Material Science and Engineering, Jiangnan University, Wuxi 214122, China |
| LIU Ya-yuan | School of Material Science and Engineering, Jiangnan University, Wuxi 214122, China |
| LI Xiao-jie | School of Material Science and Engineering, Jiangnan University, Wuxi 214122, China |
| LIU Xiao-ya | School of Material Science and Engineering, Jiangnan University, Wuxi 214122, China |
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| 中文摘要: |
| 目的 研究电泳沉积条件及光交联对氧化石墨烯与胶束共沉积制备的生物传感涂层形貌、性能的影响。方法 首先通过光敏单体甲基丙烯酸羟乙酯（HEMA）改性生物大分子γ-聚谷氨酸制备可光交联的大分子γ-PGA-HEMA。然后，将光敏大分子与辣根过氧化酶（HRP）在溶液中静电自组装制备功能性纳米粒子溶液，利用纳米粒度分析仪、透射电子显微镜（TEM）对纳米粒子的粒径和形貌进行表征。随后，诱导复合纳米组装体与前驱体氧化石墨烯（GO）共组装制备多组分复合沉积液。最后，在上述复合沉积液的基础上进行电化学还原GO，控制不同沉积条件，通过电泳沉积法在玻碳电极表面制备具有特异性识别功能的生物传感涂层，利用扫描电子显微镜（SEM）、电化学工作站等研究了电泳沉积条件及光交联对涂层形貌及性能的影响。结果 当沉积电压为1.5 V，沉积时间为120 s，GO质量浓度为0.1 mg/mL时，电泳沉积制备的涂层表面光滑，具有较好的致密性和均一性。光交联后，涂层的致密性和稳定性进一步提高，此时该涂层对过氧化氢（H2O2）具有良好的电流响应。结论 电泳沉积复合共组装胶束制备光交联涂层过程中，沉积时间、沉积电压、GO含量均存在最优值，最优值下制备的涂层结构完整，致密性最好。光交联可进一步提高涂层的稳定性，生物传感性能最好。 |
| 英文摘要: |
| The work aims to study effects of electrophoretic deposition condition and photo-cross linking on morphology and properties of GO/NPs coatings prepared by co-deposition. Firstly, hydroxyethyl methylacrylate (HEMA) was covalently conjugated to the biomacromolecule γ-Polyglutamic acid (γ-PGA) to obtain photo-cross linkable biopolymer γ-PGA-HEMA. Then, γ-PGA-HEMA was used to immobilize the molecular recognition unit horseradish peroxidase (HRP) by electrostatic self-assembly to prepare functional nanoparticles solution. The particle size and morphology of micelles were characterized with nanometer particle size analyzer and TEM. The composite nano-assembly was induced to be co-assembled with GO to prepare multi-component composite deposition solution. The HRP@γ-PGA-HEMA/rGO biological sensing coating was obtained on glass carbon electrode surface by electrophoretic deposition through electrochemical reduction of GO into graphene and control of deposition conditions. Effects of electrophoretic conditions and photo-cross linking on morphology and properties of the coating were investigated by SEM and electrochemical workstation. When electrode position voltage was 1.5 V, electrode position time was 120 sand the GO concentration was 0.1 mg/mL, the coating prepared by electrophoretic deposition exhibited good smoothness, density and uniformity. After photo-cross linking, the compactness and stability of the coating were further improved. The obtained coating showed a high response current to hydrogen peroxide (H2O2). During, the preparation of photo-cross linking coating by electrophoretic deposition composite co-assembly, there is an optimum value for deposition time, deposition voltage and GO content, which endows the electrophoretic coating with complete structure density. The photo-cross linking can further improve the stability of the coating and has the best biosensing performance. |
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